Internal Capsid-Pressure Dependence of Viral Infection by Phage Lambda

Influence of internal capsid pressure on viral infection by phage lambda.

Ejection of the genome from the virus, phage lambda, is the initial step in the infection of its host bacterium. In vitro, the ejection depends sensitively on internal pressure within the virus capsid; however, the in vivo effect of internal pressure on infection of bacteria is unknown. Here, we use microfluidics to monitor individual cells and determine the temporal distribution of lysis due t...

Control of Viral Messenger Rna after Lambda Phage Infection and Induction.

Determination of cell fate selection during phage lambda infection.

Bacteriophage lambda infection of Escherichia coli can result in distinct cell fate outcomes. For example, some cells lyse whereas others survive as lysogens. A quantitative biophysical model of lambda infection supports the hypothesis that spontaneous differences in the timing of individual molecular events during lambda infection leads to variation in the selection of cell fates. Building fro...

Novel fold and capsid-binding properties of the lambda-phage display platform protein gpD.

The crystal structure of gpD, the capsid-stabilizing protein of bacteriophage lambda, was solved at 1.1 A resolution. Data were obtained from twinned crystals in space group P21 and refined with anisotropic temperature factors to an R-factor of 0.098 (Rfree = 0. 132). GpD (109 residues) has a novel fold with an unusually low content of regular secondary structure. Noncrystallographic trimers wi...

Viral Infection Induces Dependence of Neuronal M

Inhibitory M 2 muscarinic receptors on parasympathetic nerve endings in the lungs decrease release of acetylcholine, inhibiting vagally induced bronchoconstriction. Neuronal M 2 receptor function can be studied using selective agonists and antagonists such as pilocarpine and gallamine. In pathogenfree guinea pigs indomethacin (1 mg/kg) did not alter the effect of either gallamine or pilocarpine...